Gene Transfer into Retinoblastoma Cells
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چکیده
منابع مشابه
729-electric Gene Transfer into Culture Cells *
The electric field method of gene transfer via electroporation of cell membranes is gaining importance in cellular and molecular biology. In particular the electroporation technique provides the low copy number transfection required fqr genetic studies. As demonstrated with mouse lyoma cells, with lymphoid cell lines and with plant protoplast, the electric method has appreciable advantages comp...
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This article reviews 1) the use of gene transfer methods to genetically manipulate hematopoietic stem cell targets, 2) recent advances in technology that are addressing problems that have prevented widespread successful translation of gene transfer approaches for the cure of disease, and 3) recent regulatory issues related to human gene therapy trials. In Section I, Dr. Nienhuis describes the u...
متن کاملUltrasound-mediated gene transfer into neuronal cells.
A new field of gene transfer is emerging as a simple, effective means to drive the expression foreign genes in cells: ultrasound-mediated gene transfer or sonoporation. We report here that sonoporation is an effective means of gene transfer for cultured neurons, a cell type that has been difficult to transfect. Neuronal cell types that are effectively sonoporated include chick retinal neurons, ...
متن کاملGene transfer into rat heart-derived endothelial cells.
OBJECTIVE Progressive graft arteriosclerosis is responsible for the majority of late deaths in cardiac transplant recipients. Despite many investigations, the pathogenesis of this disease remains undetermined and its control inadequate. A somatic gene transfer during the cold ischemic time and thus before transplantation might be a new therapeutic tool. This approach allows a long incubation ti...
متن کاملSide effects of retroviral gene transfer into hematopoietic stem cells.
Recent conceptual and technical improvements have resulted in clinically meaningful levels of gene transfer into repopulating hematopoietic stem cells. At the same time, evidence is accumulating that gene therapy may induce several kinds of unexpected side effects, based on preclinical and clinical data. To assess the therapeutic potential of genetic interventions in hematopoietic cells, it wil...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99263bm17